Biochemistry Laboratory

The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context…
Course Description
The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing. This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format. Acknowledgments Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan. — Course material by Dr. Elizabeth Vogel Taylor, MIT OpenCourseWare, used under CC BY-NC-SA 4.0. Original: https://ocw.mit.edu/courses/5-36-biochemistry-laboratory-spring-2009/
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